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TaKaRa
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Genmed Inc
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Millipore
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Millipore
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TaKaRa
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Journal: Cell Death & Disease
Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways
doi: 10.1038/s41419-021-04221-6
Figure Lengend Snippet: A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.
Article Snippet: The activity of Src kinase was measured according to the instructions of
Techniques: Activity Assay, Western Blot, Control
Journal: Cell Death & Disease
Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways
doi: 10.1038/s41419-021-04221-6
Figure Lengend Snippet: Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.
Article Snippet: The activity of Src kinase was measured according to the instructions of
Techniques: Phospho-proteomics, Protein-Protein interactions, Activity Assay, Blocking Assay